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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク TOPO cloning ： ウィキペディア英語版 TOPO cloning TOPO cloning is a molecular biology technique in which DNA fragments amplified by Taq polymerase are cloned into specific vectors without the requirement for DNA ligases. The Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3'-end of the PCR products. This characteristic is exploited in TOPO-cloning〔(【引用サイトリンク】 title=The technology behind TOPO® Cloning )〕 == Principle == The technique utilises the inherent biological activity of DNA topoisomerase I. The biological role of topoisomerase is to cleave and rejoin supercoiled DNA ends to facilitate replication. Vaccinia virus topoisomerase I specifically recognises DNA sequence 5´-(C/T)CCTT-3'. During replication, the enzyme digests DNA specifically at this sequence, unwinds the DNA and re-ligates it again at the 3' phosphate group of the thymidine base.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「TOPO cloning」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.